ENSMUSG00000027750

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

  • Note:

    • About the Coding Potential: Please check the spreadsheet in the “Results” tag to confirm the coding potential. The above figure only show the predicted coding potential. The “gene_biotype” in the result spreadsheet is from the database record. Therefore, if you find conflicts between “gene_biotype” and “codingPotential”, please trust the “gene_biotype”.

    • Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

    • For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENSMUST00000081564 ENSMUSG00000027750 Control shVgll3 Postn protein_coding protein_coding 572.873 625.8169 519.9291 7.009543 15.75483 -0.2674212 173.56832 129.25478 217.88186 4.847994 2.028939 0.7532831 0.3131333 0.20656667 0.41970000 0.213133333 1.504568e-24 1.504568e-24 FALSE TRUE
ENSMUST00000117373 ENSMUSG00000027750 Control shVgll3 Postn protein_coding protein_coding 572.873 625.8169 519.9291 7.009543 15.75483 -0.2674212 299.89550 384.89611 214.89489 5.326134 5.105997 -0.8408083 0.5144167 0.61530000 0.41353333 -0.201766667 3.483675e-19 1.504568e-24 FALSE TRUE
ENSMUST00000127452 ENSMUSG00000027750 Control shVgll3 Postn protein_coding retained_intron 572.873 625.8169 519.9291 7.009543 15.75483 -0.2674212 40.29478 46.26611 34.32346 3.776704 5.074231 -0.4306522 0.0697000 0.07383333 0.06556667 -0.008266667 9.706657e-01 1.504568e-24 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

Help

Please Click HERE to learn more details about the results of IsoformSwitchAnalyzeR.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand shVgll3 Control log2fold_Control_shVgll3
ENSMUSG00000027750 E001 1.3097790 2.279316e-02 8.320337e-01 9.854840e-01 3 54268530 54268545 16 + 0.384 0.346 -0.219
ENSMUSG00000027750 E002 34.5571004 1.192383e-02 4.331669e-01 9.086614e-01 3 54268546 54268563 18 + 1.513 1.579 0.225
ENSMUSG00000027750 E003 1244.6945700 9.365040e-05 4.394342e-01 9.111304e-01 3 54268564 54268688 125 + 3.089 3.098 0.030
ENSMUSG00000027750 E004 804.8957724 9.247318e-05 8.658855e-01 9.889212e-01 3 54270048 54270146 99 + 2.906 2.904 -0.006
ENSMUSG00000027750 E005 685.9204860 8.229407e-05 8.191243e-01 9.841109e-01 3 54273325 54273389 65 + 2.837 2.834 -0.009
ENSMUSG00000027750 E006 1917.1650714 4.004414e-04 4.662977e-01 9.186440e-01 3 54274922 54275079 158 + 3.286 3.278 -0.028
ENSMUSG00000027750 E007 2265.3000392 6.472872e-04 6.285174e-01 9.559579e-01 3 54276570 54276734 165 + 3.350 3.358 0.025
ENSMUSG00000027750 E008 2688.5139852 3.160908e-04 8.642705e-01 9.889212e-01 3 54277619 54277765 147 + 3.429 3.428 -0.004
ENSMUSG00000027750 E009 2293.0011122 4.925715e-04 4.381386e-01 9.109772e-01 3 54278252 54278393 142 + 3.365 3.355 -0.033
ENSMUSG00000027750 E010 3110.2950835 3.116243e-04 2.456298e-01 8.044216e-01 3 54279425 54279637 213 + 3.485 3.497 0.041
ENSMUSG00000027750 E011 2415.2449281 4.882182e-04 1.213216e-01 6.697596e-01 3 54280191 54280325 135 + 3.371 3.391 0.068
ENSMUSG00000027750 E012 2256.7103490 1.195214e-03 3.736990e-01 8.783467e-01 3 54281126 54281274 149 + 3.361 3.345 -0.053
ENSMUSG00000027750 E013 2758.0222298 9.949694e-05 2.367740e-01 8.044216e-01 3 54282439 54282575 137 + 3.444 3.435 -0.030
ENSMUSG00000027750 E014 2345.9431832 6.030575e-05 2.797446e-01 8.249186e-01 3 54283458 54283588 131 + 3.364 3.373 0.030
ENSMUSG00000027750 E015 2150.1128084 5.520005e-05 9.092850e-01 9.963212e-01 3 54284111 54284241 131 + 3.332 3.331 -0.002
ENSMUSG00000027750 E016 1801.7220743 3.359369e-04 7.082516e-02 5.733652e-01 3 54284332 54284434 103 + 3.243 3.265 0.073
ENSMUSG00000027750 E017 2032.1181982 1.402707e-04 2.401979e-04 1.569740e-02 3 54284586 54284653 68 + 3.287 3.323 0.118
ENSMUSG00000027750 E018 0.4941893 2.144694e-02 6.287478e-02   3 54284981 54284981 1 + 0.315 0.000 -11.078
ENSMUSG00000027750 E019 1410.1234559 2.701853e-04 8.804241e-05 6.912216e-03 3 54284982 54285027 46 + 3.122 3.170 0.161
ENSMUSG00000027750 E020 1069.7892399 6.361826e-04 5.588828e-02 5.175441e-01 3 54285905 54287832 1928 + 3.045 3.014 -0.101
ENSMUSG00000027750 E021 589.4919733 8.827242e-04 8.575597e-40 9.119757e-37 3 54287833 54287913 81 + 2.896 2.622 -0.912
ENSMUSG00000027750 E022 1547.7139582 1.232747e-03 7.786561e-02 5.903764e-01 3 54287914 54290527 2614 + 3.206 3.173 -0.112
ENSMUSG00000027750 E023 26.3343668 7.169376e-04 3.432860e-01 8.713685e-01 3 54290528 54290529 2 + 1.468 1.405 -0.219
ENSMUSG00000027750 E024 258.7447982 1.241341e-04 1.300339e-01 6.870645e-01 3 54290530 54290808 279 + 2.431 2.397 -0.112
ENSMUSG00000027750 E025 2309.3782121 9.021602e-05 3.137512e-01 8.521167e-01 3 54290809 54290898 90 + 3.358 3.366 0.030
ENSMUSG00000027750 E026 2107.6582360 2.304932e-04 8.648835e-03 2.027537e-01 3 54291905 54291994 90 + 3.308 3.335 0.092
ENSMUSG00000027750 E027 11.3791725 1.778539e-03 7.903567e-01 9.802084e-01 3 54291995 54292133 139 + 1.104 1.079 -0.093
ENSMUSG00000027750 E028 2672.3980008 3.635574e-04 4.551052e-04 2.681924e-02 3 54292629 54292706 78 + 3.404 3.444 0.132
ENSMUSG00000027750 E029 851.1569786 8.110227e-04 8.946294e-53 1.308172e-49 3 54293092 54293175 84 + 3.059 2.775 -0.945
ENSMUSG00000027750 E030 227.9570875 1.259804e-04 2.271891e-03 8.570028e-02 3 54296310 54296759 450 + 2.396 2.323 -0.242
ENSMUSG00000027750 E031 1931.4407590 8.503246e-04 5.087442e-01 9.308299e-01 3 54296760 54296801 42 + 3.279 3.290 0.039
ENSMUSG00000027750 E032 9639.5819460 2.181622e-04 9.167094e-02 6.249223e-01 3 54297683 54298458 776 + 3.976 3.988 0.039

Help

Please Click HERE to learn more details about the results from DEXseq.