ENSMUSG00000035325

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

  • Note:

    • About the Coding Potential: Please check the spreadsheet in the “Results” tag to confirm the coding potential. The above figure only show the predicted coding potential. The “gene_biotype” in the result spreadsheet is from the database record. Therefore, if you find conflicts between “gene_biotype” and “codingPotential”, please trust the “gene_biotype”.

    • Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

    • For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENSMUST00000182433 ENSMUSG00000035325 Control shVgll3 Sec31a protein_coding protein_coding 139.8726 170.3996 109.3456 1.798215 3.063272 -0.6399795 27.727106 36.369998 19.084214 2.501939 1.0053718 -0.9300099 0.19375000 0.21316667 0.17433333 -0.0388333333 0.60258140 6.853627e-20 FALSE  
ENSMUST00000182569 ENSMUSG00000035325 Control shVgll3 Sec31a protein_coding retained_intron 139.8726 170.3996 109.3456 1.798215 3.063272 -0.6399795 10.934947 13.373128 8.496766 0.981479 0.9442533 -0.6537326 0.07836667 0.07853333 0.07820000 -0.0003333333 1.00000000 6.853627e-20 TRUE  
ENSMUST00000182664 ENSMUSG00000035325 Control shVgll3 Sec31a protein_coding retained_intron 139.8726 170.3996 109.3456 1.798215 3.063272 -0.6399795 7.137807 8.122407 6.153207 1.177858 0.2340182 -0.4000012 0.05198333 0.04753333 0.05643333 0.0089000000 0.97066567 6.853627e-20    
ENSMUST00000182812 ENSMUSG00000035325 Control shVgll3 Sec31a protein_coding protein_coding 139.8726 170.3996 109.3456 1.798215 3.063272 -0.6399795 31.786637 34.411697 29.161578 1.092079 1.9846058 -0.2387548 0.23406667 0.20200000 0.26613333 0.0641333333 0.03536408 6.853627e-20 FALSE  
MSTRG.17850.1 ENSMUSG00000035325 Control shVgll3 Sec31a protein_coding   139.8726 170.3996 109.3456 1.798215 3.063272 -0.6399795 53.062910 64.643622 41.482197 2.015074 1.0125548 -0.6398912 0.37960000 0.37973333 0.37946667 -0.0002666667 1.00000000 6.853627e-20 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

Help

Please Click HERE to learn more details about the results of IsoformSwitchAnalyzeR.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand shVgll3 Control log2fold_Control_shVgll3
ENSMUSG00000035325 E001 1.323909 1.531358e-02 9.341328e-01 0.999634451 5 100509508 100509508 1 - 0.349 0.365 0.091
ENSMUSG00000035325 E002 1.485262 1.083402e-02 7.412570e-01 0.972900143 5 100509509 100509512 4 - 0.423 0.365 -0.323
ENSMUSG00000035325 E003 1045.604601 1.252887e-03 9.398510e-02 0.629009162 5 100509513 100509866 354 - 2.989 3.022 0.109
ENSMUSG00000035325 E004 504.378085 1.114016e-04 9.100321e-01 0.996401690 5 100509867 100510004 138 - 2.693 2.692 -0.003
ENSMUSG00000035325 E005 358.707876 1.286814e-03 5.554550e-01 0.939560912 5 100510005 100510069 65 - 2.535 2.551 0.054
ENSMUSG00000035325 E006 163.925124 2.563249e-04 2.712622e-01 0.818292058 5 100510070 100510089 20 - 2.187 2.219 0.110
ENSMUSG00000035325 E007 349.222045 1.311757e-04 1.561152e-01 0.725039829 5 100511236 100511307 72 - 2.516 2.545 0.097
ENSMUSG00000035325 E008 67.026309 7.211182e-03 9.176566e-01 0.997686457 5 100511308 100511686 379 - 1.817 1.825 0.026
ENSMUSG00000035325 E009 624.986921 2.320297e-04 9.843239e-01 1.000000000 5 100511687 100511806 120 - 2.785 2.786 0.004
ENSMUSG00000035325 E010 37.678874 3.984265e-03 3.525322e-01 0.875176952 5 100511807 100512129 323 - 1.612 1.553 -0.200
ENSMUSG00000035325 E011 186.254884 1.419103e-04 3.125747e-02 0.398695087 5 100513590 100513612 23 - 2.295 2.239 -0.188
ENSMUSG00000035325 E012 996.081895 3.827900e-04 9.008272e-02 0.620338680 5 100513613 100513853 241 - 3.002 2.979 -0.076
ENSMUSG00000035325 E013 291.764562 6.151838e-04 2.023992e-01 0.776869603 5 100516153 100516211 59 - 2.474 2.444 -0.099
ENSMUSG00000035325 E014 4.763883 3.885116e-03 9.602471e-02 0.631703792 5 100517455 100517493 39 - 0.589 0.832 1.007
ENSMUSG00000035325 E015 9.381137 2.250087e-03 2.392901e-02 0.346274667 5 100521239 100521283 45 - 0.830 1.090 0.974
ENSMUSG00000035325 E016 68.446367 2.992017e-04 1.356914e-05 0.001443016 5 100521284 100521574 291 - 1.699 1.898 0.674
ENSMUSG00000035325 E017 529.317407 8.544847e-05 6.047378e-01 0.953021573 5 100523067 100523190 124 - 2.708 2.717 0.030
ENSMUSG00000035325 E018 30.237737 1.100164e-02 4.862356e-01 0.925384695 5 100523820 100523912 93 - 1.449 1.506 0.195
ENSMUSG00000035325 E019 593.187781 1.153789e-03 3.495470e-02 0.423252920 5 100526687 100526860 174 - 2.790 2.746 -0.148
ENSMUSG00000035325 E020 371.944500 1.078441e-04 4.517084e-01 0.913499868 5 100529019 100529192 174 - 2.552 2.567 0.051
ENSMUSG00000035325 E021 246.100771 1.267326e-04 1.567761e-01 0.725988736 5 100530621 100530737 117 - 2.401 2.369 -0.107
ENSMUSG00000035325 E022 118.031549 2.074399e-04 4.834016e-03 0.139625493 5 100530738 100530766 29 - 2.119 2.026 -0.310
ENSMUSG00000035325 E023 260.294546 1.034828e-03 1.439844e-01 0.709064165 5 100531656 100531782 127 - 2.429 2.391 -0.127
ENSMUSG00000035325 E024 351.892347 1.062615e-04 4.390992e-01 0.911130350 5 100532983 100533161 179 - 2.546 2.531 -0.047
ENSMUSG00000035325 E025 201.338619 1.013824e-03 5.140153e-01 0.931492890 5 100533987 100534062 76 - 2.283 2.303 0.067
ENSMUSG00000035325 E026 191.906864 1.452165e-04 9.805395e-01 1.000000000 5 100536393 100536470 78 - 2.275 2.275 0.001
ENSMUSG00000035325 E027 138.769266 1.796144e-04 9.020959e-01 0.995632253 5 100536927 100536965 39 - 2.132 2.137 0.016
ENSMUSG00000035325 E028 165.636374 2.412828e-04 5.595494e-01 0.941104672 5 100538618 100538692 75 - 2.200 2.218 0.059
ENSMUSG00000035325 E029 316.259493 2.287254e-03 2.337167e-01 0.803782806 5 100540254 100540487 234 - 2.467 2.505 0.129
ENSMUSG00000035325 E030 272.481745 1.213058e-03 6.665261e-01 0.959214943 5 100540722 100540874 153 - 2.419 2.431 0.042
ENSMUSG00000035325 E031 294.721845 1.261302e-04 8.098178e-01 0.983262668 5 100541062 100541223 162 - 2.457 2.463 0.020
ENSMUSG00000035325 E032 181.417318 2.423869e-04 2.023998e-01 0.776869603 5 100543661 100543760 100 - 2.228 2.264 0.121
ENSMUSG00000035325 E033 230.862615 1.330181e-04 7.667010e-01 0.977990679 5 100547206 100547348 143 - 2.350 2.358 0.027
ENSMUSG00000035325 E034 0.000000       5 100549302 100549619 318 -      
ENSMUSG00000035325 E035 288.064760 1.223818e-04 6.630029e-01 0.958173263 5 100550074 100550214 141 - 2.444 2.454 0.035
ENSMUSG00000035325 E036 290.400258 6.518596e-04 6.471381e-01 0.956687053 5 100551874 100551969 96 - 2.447 2.459 0.041
ENSMUSG00000035325 E037 350.538475 1.411969e-03 8.421845e-01 0.986771512 5 100553303 100553501 199 - 2.532 2.538 0.021
ENSMUSG00000035325 E038 254.632130 1.329691e-04 6.639045e-02 0.555795647 5 100555016 100555139 124 - 2.422 2.381 -0.137
ENSMUSG00000035325 E039 1.476365 1.037437e-02 7.305883e-01 0.971405081 5 100555541 100555892 352 - 0.349 0.411 0.354
ENSMUSG00000035325 E040 183.955963 1.513325e-04 5.271063e-01 0.935744993 5 100555893 100555972 80 - 2.245 2.263 0.060
ENSMUSG00000035325 E041 12.700706 7.467591e-03 6.649116e-01 0.958488725 5 100555973 100557471 1499 - 1.151 1.109 -0.153
ENSMUSG00000035325 E042 7.248643 2.511487e-03 7.935721e-01 0.980585917 5 100563270 100563772 503 - 0.925 0.894 -0.115
ENSMUSG00000035325 E043 30.134109 1.343004e-02 1.449456e-01 0.710054028 5 100563773 100563961 189 - 1.403 1.527 0.427
ENSMUSG00000035325 E044 140.639939 1.641665e-04 8.603039e-02 0.611783295 5 100563962 100564138 177 - 2.171 2.120 -0.172

Help

Please Click HERE to learn more details about the results from DEXseq.